Method for detecting biomacromolecule based on magnetic separation-quantum dot immunofluorescence sensing and reagent preparation method

The invention provides a method for detecting a biomacromolecule based on magnetic separation-quantum dot immunofluorescence sensing and a reagent preparation method. The method is characterized by comprising the following steps: with superparamagnetic nano beads MBs as carriers, coupling the carriers with an antibody Ab1 of a biomacromolecule to be detected to prepare a capturing antibody MB-Ab1; coupling a quantum dot with an antibody Ab2 of the biomacromolecule to be detected to prepare a quantum dot fluorescence probe QDs-Ab2; enriching the biomacromolecule Ag to be detected in a test sample by using the MB-Ab1 to prepare a serial compound MB-Ab1-Ag; reacting the MB-Ab1-Ag with the QDs-Ab2 to generate an MB-Ab1-Ag-Ab2-QDs and carrying out magnetic separation to separate the MB-Ab1-Ag-Ab2-QDs from a liquid-phase QDs-Ab2 immunofluorescence probe; and measuring QDs-Ab2 fluorescence intensity X in a liquid phase and indirectly measuring C by utilizing an inverse relation represented between the concentration C of the biomacromolecule to be detected and the X. The method is simple and convenient to operate, good in stability and high in detection sensitivity.