In-silico analysis for RNA-interference mechanism of α-synuclein to treat Parkinson's disease

Parkinson's Disease PD causing mutations in α-synuclein gene are ALA30PRO, GLU46LYS and ALA53THR. The conformational changes in proteins with respect to all the three mutations were analysed. These were used to predict the structures of Short Interfering RNA siRNA antisense strand and siRNA region. The siRNA binds with the argonaute protein forming RNA Induced Silencing Complex RISC. Then, siRNA antisense-strand was attached to RISC. The structure of dicer RNase-III-enzyme cleaves double-stranded RNA dsRNA into two siRNA-strands. Incorporation of single siRNA-strand into RISC guides to pair with the complementary α-synuclein target-messenger RNA mRNA thereby enabling it to cleave the target.