[Expressions of heme oxygenase-1 and apoptosis-modulating proteins in peri-hematoma cortex after intracerebral hemorrhage in human being].

Objective To observe the expression of heme oxygenase-1 (HO-1) and Bcl-2,an apoptosis-modulating protein,in the neurons surrounding the hematoma in human being.Methods Specimens of cerebral cortex tissue 1-3 cm around the hemorrhagic focus with the size of 2.0 cm×1.5 cm×0.3 cm were collected during autopsy from 39 patients,17 males and 22 females,aged 62.8 (36-84),who died from intracerebral hemorrhage 2-10 h,17-30 h,36-96 h,120-216 h,or 240-408 h before.Specimens of brain tissue of the same size at the opposite side were collected as controls.Immunohistochemistry was used to examine the expression of HO-1 and Bcl-2 protein.Results (1)Expression of HO-1 could be detected in the specimens of the 2 h group,increased in the specimens of the 2-10 h group [(5.1±2.0)/HP],reached the peak in the 17-30 h group [(11.3±0.9)/HP],then began to decrease in the specimens of the 240-408 h group [(6.4±0.6)/HP] (F=42.80,P0.001).The HO-1 expression of the control group remained negative at any time-point.(2)Expression of Bcl-2 could be detected in the specimens of the 2-10 h group [(4.2±1.7)/HP] ,was increased in the 17-30 h group [(6.6±0.5)/HP],reached the peak in the 36～ 96 h group [(8.9±1.1)/HP],then began to decrease,and was (4.7±0.6)/HP in the 240～408 h group (F=29.59,P0.001).The Bcl-2 expression remained negative at any time point in the control group.(3)The expressions of HO-1 was positively correlated with the expression of Bcl-2 (r=0.66,P 0.001).Conclusions Over-expression of HO-1 and Bcl-2 in the neurons provide a potential protection or destruction mechanism after intracerebral hemorrhage in human.