Effect of ethanol on the mRNA expression of neuropeptide receptor and apoptosis-related genes in human bone marrow mesenchymal stem cells

Objective To observe the effect of ethanol on the mRNA expression of neuropeptide or its receptor and apoptosis-reiated genes in human bone marrow mesenclymal stem cells （hBMSCs）. Meth- ods The hBMSCs were cultivated and isolated by adherence and density gradient centfifugation, and iden- tifided by flow cytometry. The hBMSCs at the third generations were randomly divided into two groups. In the experimental group, the cells were treated with 0. 09 mol/L ethanol. In the control group, the cells were nor- mally cultured without ethanol. The mRNA expression levels of Peroxisom proliferator-activeted receptor-γ （PPAR-γ）, Calcitonin gene-related peptide receptor （CGRPR）, Runt-related transcription factor （Runx2）, B cell lymphoma-2 （bel-2） and Cysteine aspartic acid specific protease-3 （ Caspase-3 ） in hBMSCs were detected by using real-time quantitative reverse transcription-polymerase chain reaction （RT-qPCR） after culture for 11 days. Results The expression levels of CGRPR, Runx2 and bcl-2 mRNA in the experimen- tal group were significantly lower than those in the control group （for CGRPR, 1.90E-01 ± 4. 12E-02 vs. 3.27E-01 ± 8. 24E-02,t = 6. 66 ,P 〈 O. 05; for Runx, 2 1.28E-01 ± 3.18E-02 vs. 2. 50E-01 ± 5.33E-02, t =8.80,P〈0.05; for bcl-2, 1.65E-01 ±3.9gE-02 vs. 2.95E-01 ±6. TgE-O2,t =7.35,P 〈0.05）, but those of PPAR-±/and Caspase-3 mRNA were increased significantly （for Caspase-3, 5.27E-01 ± 1.26E-01 vs. 3.17E-O1 ± 7.46E-02, t = 6. 39, P 〈 0. 05 ; for PPAR-γ, 2. 90E-01 ± 6. 74E-02 vs. 1.15E-01 _± 3.36E-02 ,t = 10. 38,P 〈 0. 05 ）. Conclusion The ethanol can upregulate the exprression of apoptosis-re- lated gene and adipogenic gene in hBMSCs and downregulate the expression of neuropeptide factors and an- ti-apoptosis gene, resulting in the apoptosis and adipogeneic differetiantion of the cells and inhibiting osteo-genic differentiantion. Key words: Bone marrow mesenclymal stem cells;  Ethanol;  B cell lymphoma-2;  Caspase-3