Agrobacterium -mediated Transformation of Cotton Shoot Apex with SNC1 Gene and Resistance to Cotton Fusarium Wilt in T 1 Generation

Using shoot apexes from the upland cotton varieties, named Zhong 35 and Junmian No.1 as explant, the SNC1 , suppressor of npr1-1constitutive cloned from Arabidopsis thaliana , was introduced into cotton genome by Agrobacterium -mediated transformation with the optimized transformation conditions including culture stage of the explant, infection time and co-culture time. The results showed that the transformation procedure for high transformation rate was as follows, in vitro cuture the explants one day, infecting 20 min and co-culturing two days. PCR and RT-PCR identification of T 0 and T 1 regeneration plants revealed that the SNC1 gene was successfully integrated into cotton genome and expressed in the transgenic plant. Furthermore, comparing with non-transgenic plants, the T 1 generation transgenic plants acquired the significant resistance to the inoculation of with the Fusarium oxysporum f.sp. Vasinfectum by the validation of the root-dipping method, which suggested that the SNC1 should facilitate the resistant to cotton Fusarium wilt.