연구논문 : 정각질세포주의 피부감작물질 검색 관련 효용성 분석

Alternative methods using various human or animal cell lines are under development to classify chemical ingredients to skin sensitizing or irritant chemicals. Keratinocyte cell line could be a good target cell, in that the cells are the first contact point for chemical absorption through skin, and immune reactions including inflammation are occurred through interactions among keratinocyte, Langerhans` cell, and T cells. This study introduced current research activities on development of alternative methods using keratinocyte cell lines for identifying skin sensitizers. In addition, a good laboratory practice was introduced to perform serial passage culture of HaCaT human keratinocyte cell line. Furthermore, optimal cell density and culture duration were demonstrated to determine both intracellular and extracellular levels of IL-1α, IL-6, IL-8, and IL-18 from HaCaT cells.