Serial passage

Serial passage refers to the process of growing bacteria or a virus in iterations. For instance, a virus may be grown in one environment, and then part of that virus can be removed and put into a new environment. This process is repeated with as many stages as desired, and then the final product is studied, often in comparison with the original virus.In a series of flasks in which air is maintained in a dry state…each day one suspends a thickness of fresh rabbit spinal tissue taken from a rabbit dead of rabies. Each day as well, one inoculates under the skin of a dog 1 mL of sterilized bouillion, in which has dispersed a small fragment of one of these desiccated spinal pieces, beginning with a piece most distant in time from when it was worked upon, in order to be sure that it is not at all virulent. The findings showed that quails might play an important role in the adaptation of H5N1 avian influenza viruses to mammals. Therefore, researchers should enhance surveillance of H5N1 HPAI viruses in the quail population especially in live-bird markets. Serial passage refers to the process of growing bacteria or a virus in iterations. For instance, a virus may be grown in one environment, and then part of that virus can be removed and put into a new environment. This process is repeated with as many stages as desired, and then the final product is studied, often in comparison with the original virus. This sort of facilitated transmission is often conducted in a laboratory setting, because it is interesting to observe how the virus or bacterium that is being passed evolves over the course of an experiment. In particular, serial passage can be quite useful in experiments that seek to change the virulence of a virus or other pathogen. One consequence of this is that serial passage is useful in creating vaccines, since scientists can apply serial passage and create a strain of virus that has low virulence. Serial passage can either be performed in vitro or in vivo. In the in vitro method, a virus or a strain of bacteria will be isolated and allowed to grow for a period of time. After the sample has grown for some time, part of it will be transferred to a new environment and allowed to grow for the same period of time. This process will be repeated as many times as desired.