Trajectories and nuclear arrangement of PML bodies are influenced by A-type lamin deficiency
2012
Background information Promyelocytic leukaemia (PML) bodies are
specific nuclear structures with functional significance for
acute promyelocytic leukaemia. In this study, we analysed the
trajectories of PML bodies using single-particle tracking.
Results We observed that the recovery of PML protein after
photobleaching was ATP dependent in both wild-type (wt) and
A-type lamin-deficient cells. The movement of PML bodies was
faster and the nuclear area occupied by particular PML bodies
was larger in A-type lamin-deficient fibroblasts compared with
their wt counterparts. Moreover, dysfunction of the LMNA gene
increased the frequency of mutual interactions between
individual PML bodies and influenced the morphology of these
domains at the ultrastructural level. As a consequence of
A-type lamin deficiency, PML protein accumulated in nuclear
blebs and frequently appeared at the nuclear periphery.
Conclusions We suggest that the physiological function of lamin
A proteins is important for events that occur in the
compartment of PML bodies. This observation was confirmed in
other experimental models characterised by lamin changes,
including apoptosis or the differentiation of mouse embryonic
stem cells.
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