Studies on transgenic system of Brassica napus L. hypocotyls mediated by Agrobacterium.

In an experiment using eight double-low general rapeseed cultivars(Brassica napus L.) as researched materials,with hypocotyls of 5-6 days asepsis young plant as explants and MS medium as basic medium,an efficiency regeneration system of different rapeseed hypocotyls were preliminary established by exploring eight hormone combinations.Four varieties selected as transformation materials,which behaved higher regeneration frequency,are zhongshuang 9,7,6 and Chuanyou 11.We founded their hypocotyls transgenic system by researching the interrelated factors with transform.pta + sb(401) gene was introduced into these explants.And a lot of useful transgenic plants were obtained by PCR detecting.