Polη, Polζ and Rev1 together are required for G to T transversion mutations induced by the (+)- and (−)-trans-anti-BPDE-N2-dG DNA adducts in yeast cells

Benzo[a]pyrene is an important environmental mutagen and carcinogen. Its metabolism in cells yields the mutagenic, key ultimate carcinogen 7R,8S,9S,10R-anti-benzo[a]pyrene-7,8-dihydrodiol-9, 10-epoxide, (1)-anti-BPDE, which reacts via its 10position with N 2 -dG in DNA to form the adduct (1)trans-anti-BPDE-N 2 -dG. To gain molecular insights into BPDE-induced mutagenesis, we examined in vivo translesion synthesis and mutagenesis in yeast cells of a site-specific 10S (1)-trans-antiBPDE-N 2 -dG adduct and the stereoisomeric 10R (� )-trans-anti-BPDE-N 2 -dG adduct. In wild-type