Method for preparing bifidobacterium microcapsules by using xanthan gum / chitosan / xanthan gum and application thereof

A method for preparing bifidobacterium microcapsules by using xanthan gum / chitosan / xanthan gum and application thereof. The method is as below: inoculating a bifidobacterium freeze-dried powder to an MRS broth, activating three times, collecting thalli to obtain bacterial sludge, and preparing the bacterial sludge into a bacterial suspension; mixing the bacterial suspension and xanthan gum, adding a deoxidant solution, mixing well to obtain a bifidobacterium glue solution, adding the bifidobacterium glue solution into a chitosan solution, and stirring to obtain the microcapsules; and after complete crosslinking of the microcapsules, filtering, mixing and stirring the microcapsules with a xanthan gum solution again, filtering and washing, so as to finally obtain a double-layer bifidobacterium microcapsules with viable count of 3.0-4.5*10 CFU / g. After addition of the prepared bifidobacterium microcapsules into homemade yogurt and storage for 4 weeks, the viable count is still greater than 10 CFU / g, and acidity and pH of the yogurt changed little, and the yogurt maintains unaffected quality and can effectively play its probiotic effect after drinking.