Use of endospore-forming bacteria as an active oxygen scavenger in plastic packaging materials

Abstract The incorporation of active oxygen scavengers in polymer packaging materials is essential to allow packaging of oxidation sensitive products. Opposed to the currently available chemical oxygen scavengers, systems based upon natural and biological components could have advantages towards consumer perception and sustainability. A modelsystem for a new oxygen scavenging poly(ethylene terephthalate) (PET) bottle is proposed using an endospore-forming bacteria genus Bacillus amyloliquefaciens as the active ingredient. Spores were incorporated in poly(ethylene terephthalate, 1,4-cyclohexane dimethanol) (PETG), an amorphous PET copolymer having a considerable lower processing temperature and higher moisture absorption compared to PET. To asses spore viability after incorporation, a method was optimized to extract spores from PETG using a chloroform/water mixture. Samples were also analyzed using a Live/Dead Bac Light Bacterial Viability kit. It was shown that endospores were able to survive incorporation in PETG at 210 °C. Incorporated spores could actively consume oxygen for minimum 15 days, after an activation period of 1–2 days at 30 °C under high humidity conditions. Industrial relevance The study describes a modelsystem for the use of incorporated spores genus Bacillus amyloliquefaciens as an active oxygen scavenger in PET multilayer bottles using PETG as the middle layer material. Industrially, oxygen scavengers using incorporated viable spores as the active compound could have advantages towards consumer perception, recyclability, safety, material compatibility, production costs, … compared to currently available chemical oxygen scavengers.