An intrinsically disordered region of RPN10 plays a key role in restricting ubiquitin chain elongation in RPN10 monoubiquitination

Despite being a common mechanism in eukaryotes, the process by which protein monoubiquitination is produced and regulated in vivo is not completely understood. We present here the analysis of the process of monoubiquitination of the proteasomal subunit Rpn10 (regulatory particle non-ATPase 10), involved in the recruitment of polyubiquitinated substrates. Rpn10 is monoubiquitinated in vivo by the Nedd4 (neural precursor cell expressed developmentally down-regulated 4) enzyme Rsp5 (reverses SPT-phenotype protein 5) and this modification impairs the interaction of Rpn10 with substrates, having a regulatory effect on proteasome function. Remarkably, a disordered region near the ubiquitin-interacting motif of Rpn10 plays a role in the restriction of the polyubiquitin extension activity of Rsp5. Mutations in this disordered region promote ubiquitin chain extension of Rpn10. Thus, our work sheds light on the molecular basis and the functional relevance of a type of monoubiquitination that is driven by the substrate. Moreover, we uncover a putative role for disordered regions in modulating ubiquitin–protein ligation. * CP, : core particle; CPY, : Carboxypeptidase Y; Dsk2, : dual-specificity protein kinase 2; Dss1, : deleted in split hand/split foot protein 1; DUB, : deubiquitinating enzyme; HECT, : homologous with E6-associated protein C-terminus; Nedd4, : neural precursor cell expressed developmentally down-regulated 4; Ni-NTA, : Ni2+-nitrilotriacetic acid; Pgk1, : 3-phosphoglycerate kinase; Rnf114, : ring finger 114; RP, : regulatory particle; Rpn, : regulatory particle non-ATPase; Rsp5, : reverses SPT-phenotype protein 5; Tom1, : Trigger of mitosis 1; Ubc4, : ubiquitin-conjugating enzyme 4; UBD, : ubiquitin-binding domain; UIM, : ubiquitin-interacting motif; WT, : wild-type