Generation of novel self‐incompatible Brassica napus by CRISPR/Cas9

Self-incompatibility (SI) is a genetic mechanism that prevents inbreeding in hermaphrodite angiosperms via the rejection of self-pollen. SI lines facilitate the utilization of heterosis (De Nettancourt 2001). In cruciferous plants, SI is determined by the S locus, which harbors genes encoding the stigma papilla cell-specific S-receptor kinase (SRK) and the pollen surface-localized ligand of SRK, S-locus cysteine-rich protein/S-locus protein 11 (SCR/SP11) (Schopfer et al., 1999; Suzuki et al., 1999).