Myogenic propensities of cells obtained from liver by preplating

pancreatic endocrine cell have been tried. Many studies showed that the AMSCs can be differentiated into pancreatic endocrine cells in vitro. But the rate of transdifferentiation was low. Recent studies showed that hypoxia affects proliferation and differentiation ofmesenchymal stem cells, and controls the differentiation of several cell types during development. AndHypoxia decreases the differentiation of neural precursor cells, myogenic cells, adipocyte, pancreatic beta cells from embryonic stem cells. In this study, we evaluated whether low oxygen level affects the proliferation and trans-differentiation of AMSCs. When the AMSCs cultured in 3% hypoxia condition, the AMSCs were increased cell number compared with normoxia condition. In addition, hypoxia affected into pancreatic differentiation. The expression of pancreatic transcription factors nkx6.1 were increased in hypoxia culture comparedwith normoxia control. And the amount of expressed endocrine cell marker was changed in hypoxia condition. But the expression of another pancreatic transcription factors, pdx1 or neuroD have not been changed. In our data suggested that hypoxia controls proliferation and affects trans-differentiation into pancreatic endocrine cells from AMSCs.