Crystallographic Analysis of New Psychrophilic HaloalkaneDehalogenases: DpcA from Psychrobacter cryohalolentis K5 andDmxA from Marinobacter sp. ELB17
2013
Haloalkane dehalogenases are hydrolytic enzymes with a broad
range of potential practical applications such as
biodegradation, biosensing, biocatalysis and cellular imaging.
Two newly isolated psychrophilic haloalkane dehalogenases
exhibiting interesting catalytic properties, DpcA from
Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp.
ELB17, were purified and used for crystallization experiments.
After the optimization of crystallization conditions, crystals
of diffraction quality were obtained. Diffraction data sets
were collected for native enzymes and complexes with selected
ligands such as 1-bromohexane and 1,2-dichloroethane to
resolutions ranging from 1.05 to 2.49 A.
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