High resolution surface plasmon microscopy for cell imaging
2010
We introduce a new non-labeling high resolution microscopy method for cellular imaging. This method called
SSPM (Scanning Surface Plasmon Microscopy) pushes down the resolution limit of surface plasmon resonance
imaging (SPRi) to sub-micronic scales. High resolution SPRi is obtained by the surface plasmon lauching with a
high numerical aperture objective lens. The advantages of SPPM compared to other high resolution SPRi's rely
on three aspects; (i) the interferometric detection of the back reflected light after plasmon excitation, (ii) the twodimensional
scanning of the sample for image reconstruction, (iii) the radial polarization of light, enhancing both
resolution and sensitivity. This microscope can afford a lateral resolution of - 150 nm in liquid environment
and - 200 nm in air. We present in this paper images of IMR90 fibroblasts obtained with SSPM in dried
environment. Internal compartments such as nucleus, nucleolus, mitochondria, cellular and nuclear membrane
can be recognized without labelling. We propose an interpretation of the ability of SSPM to reveal high index
contrast zones by a local decomposition of the V (Z) function describing the response of the SSPM.
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