Native plant Maireana brevifolia drives prokaryotic microbial community development in alkaline Fe ore tailings under semi-arid climatic conditions

Abstract Native pioneer plants of high environmental tolerance may be exploited as early colonisers in alkaline Fe-ore tailings to drive the development of functional prokaryotic microbial communities, which is one of the critical pedogenic processes leading to in situ soil formation in the tailings. The present study deployed high throughput Illumina Miseq sequencing, to characterise the diversity and potential functionality of prokaryotic microbial communities in the aged Fe-ore tailings and topsoils colonised by native plant species Maireana brevifolia at an Fe ore mine in Western Australia, in comparison with those in the tailings/topsoils without plants. The composition of prokaryotic microbial communities differed between the aged tailings (AT) and topsoil sites (TS). Aged tailings (AT1-AT3) contained more bacteria tolerant of alkaline/saline conditions (e.g., Alkalilimnicola sp.) and those related to Fe biogeochemical cycling (e.g., Acidiferrobacter sp., Aciditerrimonas sp.). In comparison, the prokaryotic microbial communities in the topsoil (TS) contained abundant bacteria related to N cycling (e.g., Rhizobium sp., Frankia sp.). The presence of M. brevifolia plants significantly increased the diversity of prokaryotic microbial communities in tailings and topsoil, particularly favouring the development of bacteria related to N cycling and OM degradations (e.g., Mesorhizobium sp. Paracoccus sp., Oxalicibacterium horti, and Microbacterium sp.). The variation of microbial community were mainly explained by pH, amorphous Fe, and total N, which were regulated by M. brevifolia colonisation. The beneficial roles of pioneer plants M. brevifolia in the development of prokaryotic microbial community in the alkaline Fe ore tailings may be integrated as a key factor when designing and scaling up the process of eco-engineering Fe-ore tailings into soil under semi-arid climatic conditions.