203 Successful Engraftment of Cultured Small Intestinal Epithelial Stem Cells Onto Damaged Colonic Mucosa by Heterotopic Transplantation

were established and demonstrate that rID existed in proximal and distal human tissues and maintained in the enteroids. These data support the observation that rID is maintained cell autonomously by ISCs, independent of input from the surrounding niche. Therefore, the rID is enforced by differential chromatin marks suggesting that an epigenomic state maintains the rID within ISCs. This study provides novel insight towards understanding regional differences within ISCs that will be informative to both basic science and clinical intestinal research.