HO-1在CCK-8减轻脂多糖所致的急性肺损伤中的作用

AIM: To study the role of heme oxygenase (HO)-1 in the mechanism of cholecystokinin-octapeptide (CCK-8) for attenuation of acute lung injury (ALI) induced by lipopolysaccharide (LPS) METHODS: Adult male rats were randomly divided into five groups: control group, LPS group, CCK - 8 + LPS group, LPS + Hm (hemm, HO-1 donor) group and LPS + ZnPP (zinc protoporphyrin, specific inhibitor of HO-1) group. PMN number in bronchoalveolar lavage fluid (BALF), the structure of the lung, MDA content, HO-1 activity, the expressions of HO-1 mRNA and protein in the lung were detected respectively. RESULTS: The lung injury in LPS group was observed, at the same time the numbers of PMN, the content of MDA, the activity and the expression of HO-1 were all higher than those in control group (all P＜0.05). The degree of lung injury, PMN numbers and MDA content were lower, while the activity and the expression of HO-1 in CCK - 8 + LPS and LPS + Hm group were higher than those in LPS group (all P＜0.05). However, the degree of lung injury, PMN numbers and MDA content were higher, the activity and the expression of HO-1 were lower in LPS + ZnPP than those in LPS group respectively (all P＜0.05). CONCLUSION: CCK-8 attenuates the LPS-induced ALI by means of anti-oxidation and inhibits PMN aggregation, which are both mediated by HO-1 partly.