Primer for diagnosing lung lesions caused by chrysotile and man-made mineral fiber by using RT-PCR method

The invention relates to a primer for diagnosing lung lesions caused by chrysotile and man-made mineral fiber by using an RT-PCR method. The primer comprises a primer pair 1, a primer pair 2 and a primer pair 3 which are respectively used for amplifying miR-29a, miR-29b and miR-29c, and a base sequence is showed as SEQ ID NO.4-9. An miRNA amplification primer is designed on the basis of rat lung tissue pathological mechanism expressions by building a rat dust dying model, expression situations of miR-29 family members in dust dying rat lung tissues are detected, and an effective tool of early diagnosis and screening of health hazards caused by exposure of the chrysotile and the man-made mineral fiber is provided.