The Down-regulation Effect of Liraglutide(Lira) on the Expression of Double-stranded RNA-dependent Protein Kinase-like Endoplasmic Reticulum Kinase(PERK) in βTC3 Cells Induced by Free Fatty Acids(FFA)

Objective: To investigate the expression of PERK in βTC3 cells exposed to different concentrations of FFA and the intervention effect of Lira on the expression of double-stranded RNA-dependent protein kinase-like endoplasmic reticulum kinase(PERK) induced by FFA.Methods: βTC3 cells were treated with different concentrations of FFA(0,0.125,0.25,0.5 and 1.0 mmol/L).Western Blot analysis was used to determine the expression of PERK in βTC3 cells after 24 hours.Afterwards,βTC3 cells were prein-cubated with different concentrations of Lira(0,0,0.5,1 mg/L)for 6 hours,and different concentrations of FFA(0,1,1,1 mmol/L) were then added and the cells were incubated for another 24 hours.The expression of PERK was detected.Results: ①After the cells were in-cubated with FFA of different concentrations for 24 hours,compared with the control group,the expression of PERK in βTC3 cells in the group with 1 mmol/L FFA increased(P0.05).②Compared with that in the group with 1 mmol/L FFA,the expression of PERK de-creased(P0.05) in the group with 0.5 mg/L Lira+1 mmol/L FFA and the group with 1 mg/L Lira+1 mmol/L FFA,and there was statisti-cal difference between the two groups(P0.05).Conclusion: The expression of PERK in βTC3 cells is up-regulated by administration of FFA of certain concentration,while Lira can reverse this response to some extent,partly inhibiting endoplasmic reticulum stress.