[Modulatory effect of orphanin FQ on the IL-1beta transcripts in hippocampus].

Using in situ hybridization and immuno-fluorescent double labeling method, we investigated the function of orphanin EQ on interlenkin-1β (IL-1β) transcripts in hippocampus. Intracerebroventricularly (icv) administrated IL-1β antibody reduced IL-1 and TNF-α secreted from peritoneal macrophage.The high level of IL-1β transcript in hippocampus elicited by traumatic stress was blocked by icy injection of orphanin EQ (0.55 nmol), which was reversed by orphanin EQ receptor antagonist ([phe1 (CH2-NH)Gly2]nociceptin-( 1-13)-NH2). Opioid receptor-like receptor transcripts were found in neuron, astrocyte and microglia. Based on the results, we conclude that orphanin EQ functions as a neuroimmune modulator, and provokes immune response via mediation of IL-1β derived from neuron, astrocyte and microglia in hippocampus.