Properties of hybridization isotherms upon binding of ligands on microchips

A simple thermodynamic model describing microarray oligo-target hybridization has been constructed. The relationship between the hybridization signal intensity and Gibbs free energy for oligo-target duplex formation has been considered. The behavior of this function, which we called energy hybridization isotherm, in response to target concentration change was modeled at different ratios of oligo/target concentrations. The results of modeling were compared with the relevant and currently available data from microarray adsorption experiments.