Establishment of Etv5 gene knockout mice as a recipient model for spermatogonial stem cell transplantation

Transplantation of allogeneic spermatogonial stem cells (SSCs) into the testes could generate heterologous sperm and thus produce offspring by natural breeding. This has a significant impact on the conservation of economically valuable and endangered animals, as well as to the understanding of SSCs function. In this study, we report the generation of Ets-variant gene5 ( Etv5 ) knockout (KO) mice using the CRISPR/Cas9 system. The testicular weight of Etv5 -/- mice was significantly lower compared to wild-type (WT) mice (P