Preparation method of artificial skin by taking VEGF165 gene modified hair follicle stem cells as seed cells

The present invention relates to a method of preparing genetically modified VEGF165 as the artificial skin of the seed cells hair follicle stem cells. The method comprises the steps of: 1) collecting good shape and the hair follicles in growth phase; purified follicle stem cells 2); identifying hair follicle stem cells. 3); 4) lentiviral; 5) lentiviral follicle stem cells; 6) gelatin preparation of spongy three-dimensional tissue scaffold; 7) taken before stored at 4 ℃ gelatin sponge holder, by injection cells were seeded in gelatin sponge holder and inner surface, the artificial skin obtained using the above methods. Rapid large amplification of the present invention in hair follicle stem cells as seed cells, in vitro culture, the source of a very rich, reduced immune rejection; while taking advantage of VEGF165 gene transfection modified follicle stem cells, capable of forming a new project with the expansion and contraction function the vascular system, a good solution to easily transplanted artificial skin necrosis, low survival rate issues.