A screening method for the optimal surface structure of embryonic stem cell culture using a cell culture plate comprising gradient nanopattern

The present invention provides a method of screening a surface structure suitable for the diameter (Diameter) or spacing Embryonic Stem Cell Culture (Spacing) comprises the step of the cell culture the cells in the culture vessel containing the gradient nano-pillar array that change event, the cell comprising a nano-pillar of the selected condition by the cell culture vessel, and the screening method comprises the constant increase nanopillars are uniformly arranged at intervals which decrease the nanopillars is constant with a diameter that is used in the screening method of culturing It relates to a method of culturing embryonic stem cells and without feeder cells comprising the step of culturing the cells in the container maintain a stem cell function (stemness). A cell culture vessel comprising a gap (Spacing) uniformly arranged nano-columns with a nano-pillar is reduced a constant having a diameter (Diameter) monotonically increasing of the invention is to provide a variety of surface types within a single culture vessel it may, by a single incubation is carried out under the same conditions, the change in the surface morphology can be monitored at the same time the effects on cell characteristics may be selected for optimum surface forms suitable for cell culture by using this. Method of culturing the human embryonic stem cell selected through the screening can provide a method capable of proliferation while maintaining the stem cell function without coating the outer expensive cell substrate, or the need to use feeder cells.