Expresssion and Change of SIAH1 in Rat Models of Spinal Cord Injury

Objective To observe the expression patterns of absentia homolog 1(SIAH1) in rat models of spinal cord injury,and the association with neuronal survival or apoptosis and glial proliferation was analyzed.As well as to investigate their potential biological roles in nerve injury and regeneration. Methods Sprague-Dawley(SD) rats were subjected to spinal cord injury.In the purpose of exploring the pathological and physiological significance of SIAH1,the expression patterns and distribution for SIAH1 were observed by Western blot,and tests of immunohistochemistry and immunofluorescence.Results Western blot analysis showed SIAH1 protein level was significant up-regulated at the 8th hour,1st day and 2nd day after injuried,then reduced and there was no up-regulation till the 14th day.Immunohisto-chemical analysis showed that the immunochemical staining was increased at the 1st day and reduced at the 7th day after injury.Immunoflurescence analysis showed SIAH1 were expressed in neurons,astrocytes and microglias.Conclusion The up-regulation of SIAH1 in the injured spinal cord may associate with apoptosis process of neurons and glial proliferation.