Resolution of Synthetic (+)- and (-)-Epibatidine by Chiral High Performance Liquid Chromatography and Identification of the Natural Enantiomer

Abstract Methods are described for the separation of the enantiomers (+)- and (-)-epibatidine and (+)- and (-)-N-acetylepibatidine by high performance liquid chromatography using Chiral-AGP and Chiralcel OD chiral stationary phases respectively. Comparison of synthetic material with authentic epibatidine. HC1 using retention times and UV spectra allows the unambiguous assignment of the natural product as the (+)- isomer.