The barley thaumatin-like protein Hv-TLP8 enhances resistance of oilseed rape plants to Plasmodiophora brassicae

A barley cDNA for the thaumatin-like protein Hv-TLP8 driven by the CaMV 35S promoter was introduced into oilseed rape (Brassica napus) via Agrobacterium-mediated transformation. Some transgenic lines were obtained which exhibited enhanced resistance to the clubroot pathogen (Plasmodiophora brassicae). To increase resistance, double haploid (DH) lines were generated from these transgenic plants. Of these, DH-line 189 showed stable inheritance of the transgenic trait and its associated resistance for at least three generations.