Comparative evaluation of surface micro hardness and morphology of enamel remineralization using chitosan hydrogel and APF gel: An in vitro study

Biocompatible chitosan hydrogel shows promise as a biomaterial for the prevention and treatment of defective enamel over Fluoride application. Thirty three human anterior extracted teeth were selected for study and divided into three groups: Group I: Control group, teeth etched with 37% phosphoric acid for 30 seconds. Group II: Teeth etched with 37% phosphoric acid for 30 seconds, coated with APF gel, then incubated at 37 oC in artificial saliva solution for 10 days. Group III: Teeth etched with 37% phosphoric acid for 30 seconds, coated with bonding agent and chitosan hydrogel, then incubated at 37 oC in artificial saliva solution for 10 days. After remineralization, enamel surfaces observed with scanning electron microscope for induced structural changes on enamel surface and surface micro hardness was evaluated. Results showed statistically significant difference (P