Insecticidal Effect of Transgenic Tobacco Harboring cry2Ab4 or vip3Aa11 Gene on Agrotis ypsilon

Agrotis ypsilon is a destructive pest in agricultural production.The way to improve crop resistance against Agrotis ypsilon by genetic manipulation is available.Previously,the cry2Ab4 gene and vip3Aa11 gene were cloned from Bacillus thuringiensis.Two plant expressive vectors pCS2Ab and pCSvip3A harboring cry2Ab4 gene and vip3Aa11 gene were constructed and transferred into tobacco(Nicotiana tabacum L.) NC89 by Agrobacterium mediated method.32 and 35 positive regenerated plants were obtained and confirmed by PCR,RT-PCR and Western blot,respectively.The insect-resistant capability in the transgenic tobacco plants with pCS2Ab and pCSvip3A vectors had also been detected by bioassay.The mortality of Agrotis ypsilon larvae fed with leaves from transgenic plants was above 82%,which was significantly more than the contrast.The insecticidal activity of transgenic tobacco harboring vip3Aa11 gene is higher than that of transgenic tobacco with cry2Ab4 gene.