Fluid flow-induced bending of the primary cilium triggers a distinct intraciliary calcium flux in mesenchymal stem cells

Objective Primary cilia are proposed to form a regulatory microdomain in mesenchymal stem cells (MSCs) where they have also been shown to be necessary for loading-induced osteogenic lineage commitment. The specific mechanisms mediating this are unclear. This study aims to characterise subcellular ciliary calcium signalling in response to mechanical load and identify the mediating channels in MSCs.