Relationship between human papillomavirus types and cervical diseases

Objective To investigate the human papillomavirus (HPV) types detected in patients with non-cancer, pre-cancer and cancer lesions at cervix and to analyze the oncogenesis of the various HPV types.Methods The cervical specimens from 356 patients with HPV infection were collected, Hybrid Capture Ⅱ (HCⅡ) assay was used to detect the 13 HPV-H types, and DNA flow-through hybridization genotyping technique was applied to determine the 21 HPV-DNA types. All of the patients received liquid-based cytology test (LCT) and colposcopy with multiple biopsy. According to the cytological and pathological diagnosis, the patients were divided into three groups: ① cervical cancer and high-grade squamous intraepithelial lesions (HSIL) group, including 9 cases of invasive cervical cancer, 57 CINⅢ and 53 CINⅡ; ② low-grade squamous intraepithelial lesions (LSIL) group, including 112 CINⅠ and 52 mere HPV infection; ③ 73 in normal or inflammatory group. The relationship between HPV types and different cervical diseases was analyzed. Results HPV-H was tested positive in 98.4% of HSIL patients. In normal or inflammatory, LSIL and HSIL groups, double or multiple HPV-H type infection occurred at 13.7%, 17.7% and 36.6% respectively, with HSIL group noticeably higher than the other two groups, P0.01.In this study, the prevalence of HPV types from highest to lowest order were as follow: HPV-16 (32.9%), 58 (18.8%), 52 (16.9%), 18 (9.0%), 33 (8.2%), 68 (7.3%), 11 (low risk type, 6.5%), 31 (6.2%), 53 (low risk type, 5.6%), 39 (5.3%), CP8304 (5.1%), 66(low risk type, 3.9%), 6 (2.5%), and other rare types 51,56,45,35,59,43,44,82, etc.The infection rates of HPV types 16,58,33,52 and 18 in HSIL were 56.4%, 19.3%, 16.0%, 12.6% and 8.4% respectively. The data from HCⅡ and HPV-DNA genotyping were consistent. Conclusions HPV-H is closely associated with cervical cancer. HPV types 16,58,52,18,33 are more common in cervical disease more oncogenic than other HPV types. HPV-DNA genotyping is of great importance in diagnosis and prognosis of cervical disease.