Hematopoietic Progenitor Stem Cell Homing in Mice Lethally Irradiated with Ionizing Radiation at Differing Dose Rates

Abstract Collis, S. J., Neutzel, S., Thompson, T. L., Swartz, M. J., Dillehay, L. E., Collector, M. I., Sharkis, S. J. and DeWeese, T. L. Hematopoietic Progenitor Stem Cell Homing in Mice Lethally Irradiated with Ionizing Radiation at Differing Dose Rates. Radiat. Res. 162, 48–55 (2004). It has recently been shown that specific lineage-depleted murine hematopoietic stem cells that home to the bone marrow 2 days after transplantation of ablated primary recipients are capable of long-term engraftment and repopulation of secondary recipients. We were interested in determining whether the rate at which the ablating radiation dose was delivered to the mice affected the homing of lineage-depleted stem cells to the bone marrow and/or sites of tissue damage. Fractionated, lineage-depleted donor marrow cells were isolated and labeled with the membrane dye PKH26. Recipient mice were lethally irradiated with 11 Gy ionizing radiation using varying dose rates and were immediately injected with PKH26-labeled progenitor...