Ultrathin Coatings with Change in Reactivity over Time Enable Functional In Vitro Networks Of Insect Neurons

Combining a suitable microfabrication technology with surface modification techniques provides adherent binding sites for growing cells in spatially controlled regions with a nonfouling background. [5] Such biofunctional patterns on a protein-repellent background yield geometrically confined cellular networks on artificial surfaces. This in vitro technique provides a means of systematic manipulation of the extracellular environment for investigating cell‐surface and cell‐ cell interactions. Covalent immobilization of extracellular factors also provides pattern stability for long-term cell-based experiments. [6]