Study on molecular target promoting human neural stem cells of ginsenoside Rg1 by gene chip

Objective: To screen out main molecular target promoting human neural stem cells(NSCs) of ginsenoside Rg1 by using the gene chip technology.Method: First,MTT assay was adopted to screen out the optimal concentration of Rg1-promoted NSC proliferation(120 mg·L-1).Then,on the 7th day after the Rg1-promoted NSC proliferation,the expression of target genes was observed by the gene chip technology.The most important target gene and signal transduction pathways were screened out through the data calculations.Result: On the 7th day after the Rg1-promoted NSC proliferation,obtained 440 differential genes,266 significantly up-regulated genes and 174 significantly down-regulated genes.HES1 gene,CAMP(cyclic adenosine monophosphate)-PKA(protein kinase A) and PI3K(phosphatidylinositol 3 kinase)-AKT signal transduction pathways were closely related to the NSC proliferation.Conclusion: The differentially expressed genes screened out by gene chip may provide new clues for studies on molecular mechanism of ginsenoside Rg1-promoted NSCs proliferation.