Transgenic plants : methods and protocols

1. Repurposing macromolecule delivery tools for plant genetic modification in the era of precision genome engineering 2. The use of an automated platform to assemble multigenic constructs for plant transformation 3. Ensifer-mediated transformation (emt) of rice (monocot) and oilseed rape (dicot) 4. Setaria viridis as a model plant for functional genomic studies in C4 crops 5. Transient transformation using particle bombardment for gene expression analysis 6. Maize transformation using the morphogenic genes babyboom and wuschel2 7. Efficient and fast production of transgenic rice plants by agrobacterium-mediated transformation 8. Protocol for Agrobacterium-mediated transformation and transgenic plant production of switchgrass 9. Biolistic transformation of wheat 10. Mesophyll protoplasts and PEG-mediated transfections: Transient assays and generation of stable transgenic canola plants 11. A Unified Agrobacterium-mediated transformation protocol for alfalfa (Medicago sativa l.) and medicago truncatula 12. Poplar transformation 13. The genetic transformation of sweet orange (Citrus sinensis L. Osbeck) for enhanced resistance to citrus canker 14. Genetic modification of grapevine embryogenic culture 15. Agrobacterium-mediated transformation of Solanum tuberosum L., potato 16. Agrobacterium tumefaciens-mediated transformation of tomato 17. DNA break repair in plants and its application for genome engineering 18. Gene stacking in plants through the application of site-specific recombination and nuclease activity 19. CRISPR/Cas9 for mutagenesis in rice 20. Plant biotechnology applications of zinc fingers technology 21. Overview of biotechnology-derived herbicide tolerance and insect resistance traits in plant agriculture 22. Developing transgenic agronomic traits for crops: targets, methods and challenges 23. Transgenic and genome editing approaches for modifying plant oils 24. Summary of molecular analysis methods for characterizing transgenic events 25. Detection of transgenic proteins by immunoassays 26. Systematic evaluation of field crop performance using modern phenotyping tools and techniques