MDAH- 2774 over kanser kök hücrelerinde kemoterapi sonrası apoptotik etkilerin incelenmesi

Aim: A major problem in the treatment of ovarian cancer, which has the highest mortality rates among gyneacological malignancies, is recurrence and chemoresistance. In the cancer tissue, this population of long-lived cells with extraordinary expansion potential has been called tumor initiating cells or cancer stem cells (CSCs). It is thought that CSCs are responsible for these aggressive and recurrent tumors. These cells that differentiate from normal stem cells and form tumor tissue are isolated in-vitro via the Multicellular Tumor Spheroid Model. In this study, Gemcitabine (2′2′-difluorodeoxycytidine, Gemzar®), which is a deoxycytidine analog with excellent antitumor activity has been used for its anti-apoptotic effects.. Material and Methods: CSC isolation was been performed in-vitro three dimensional cell cultures from MDAH-2774 ovarian CSC line and acts of Gemcitabine were determined. CSCs treated at 24th and 96th hours were analyzed for apoptotic alterations with p-53, bcl-2, and caspase-3 immunoreactivity. Results: For the Gemcitabine group, p53 immunoreactivity increased at 24th and 96th hours and bcl-2 decreased at the 24th hour while an apparent increase was determined at 96th hours. Caspase-3 decreased for both groups at 24/96 hours while caspase-3 revealed decrease at 96th hours. Conclusion: Increase in p53 immunoreactivity as well as decrease in bcl-2 display that drug activity is through these pathways while caspase-3 pathway is not active. Therefore, the expected increase could not be assessed. Until now, drug sensitivity tests and effects on CSCs have been evaluated with monolayer cell culture systems. It is appropriate to evaluate these studies with CSCs isolation technique.