Cultivation and identification of adipose tissue-derived mesenchymal stem cells from rabbits

Mesenchymal stem cells isolated from rabbit inguinal fat pads were digested with collagenase type I,and their molecular characteristics and multi-lineage potential were identified by immunohistochemical methods and induction in vitro.In results,rabbit adipose tissue derived mesenchymal stem cells could express CD29,CD44 and CD105,whereas they did not express CD34,CD45 or HLA-DR.They had the(ability) to differentiate into adipocytes,neurocytes and osteoblasts.The results provided significant evidence that there are multipotential mesenchymal stem cells in rabbit adipose tissue.