897. Na,K-ATPase β1-Mediated Selective Gene Transfer into Neurons

Neuron-selective gene transfer is an attractive therapeutic strategy for neurological disorders. Selective gene transfer requires high efficiency as well as tissue specificity of the target molecule employed. Selection of an optimal target molecule is critical. Here, we contrived the hybridoma library screening to identify optimal target molecules for neuron selective gene transfer using fiber mutant adenoviruses as targeting tools. We generated genetically modified adenoviruses incorporating Z33, an IgG Fc-binding motif from Staphylococcus protein A, at the fiber (Adv-FZ33 and Adv-FdZ). The Z33 vectors were capable of infecting cells through use of linked antibodies against surface antigen molecules.