Detection of HPV DNA in genital condylomata acuminata in female prepubertal children

Abstract In situ DNA hybridization using biotin-labeled probes for HPV types 6, 11, 16, and 18 was performed on paraffin-embedded anogenital condylomata acuminata from four children. Three, age 9 years, 22 months, and 10 months, were positive for human papillomavirus (HPV) 6. The first two were also positive for HPV 11. A fourth child, age 6 years, was negative for all four HPV types. The mother of the 10-month-old infant had a condylomatous infection of the cervix as detected by cytology, histology, and colposcopy but dot blot hybridization of cervical tissue was negative for the four HPV types. These cases form the nucleus of a long-term prospective study of anogenital HPV infection in children, as the significance of various HPV types of infection in differing anogenital locations is poorly understood.