Protein phosphorylation : a practical approach

Preface 1. Study of protein phosphorylation in intact cells 2. Analysis of signal transduction pathways using protein kinase inhibitors and activators 3. Analysis of signal transduction pathways using proteinserine/threonine phosphatase inhibitors 4. Analysis of signal transduction pathways using molecular biological manipulations 5. Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates 6. Phosphorylation site analysis by mass spectrometry 7. Assay and purification of protein (serine/threonine) phosphatases 8. [chapter title missing] 9. Identification of substrates for protein-tyrosine phosphatases 10. Assay and purification of protein-serine/threonine kinases 11. Assay of protein kinases and phosphatases using specific peptide substrates 12. Cloning and expression of cDNAs encoding protein kinase subunits 13. Interaction cloning of protein kinase partners 14. Identifying protein kinase substrates by expression screening with solid-phase phosphorylation 15. Analysis of protein kinase interactions using the two-hybrid method 16. Analysis of protein kinase interactions using biomolecular interaction analysis 17. Analysis of protein kinase specificity using orientated peptide libraries 18. Crystallization of protein kinases and phosphatases Index