IN VITRO ANTIPLASMODIAL AND ANTILEISHMANIAL ACTIVITIES OF FLAVONOIDS FROM ANOGEISSUS LEIOCARPUS (COMBRETACEAE) Research Article

Anogeissus leiocarpus Guill. & Perr. (Combretaceae) is an African indigenous tree. It has been of interest to researchers because it is used in Ivory Coast as antimalaria remedy. The in vitro antiplasmodial and antileishmanial activities of the leaf and it major constituents are investigated her for the first time. Chemical composition of ethyl acetate crude extract was analyzed by NMR (1D and 2D), LC-ESI-MS and HPLC reverse phase methods. Results demonstrated that ethyl acetate crude extract from A. leiocarpus presented in vitro antiplasmodial and antileismanial activities (IC50: 10 and 25 µg/ml respectively). Eight flavonoids were isolated, among which Procyanidin B2 (8) and Quercetin (3), that showed antiplasmodial activity with IC50 value of 5.3 and 6.6 µM respectively. With the antileishmanial activity, the best IC50 value was obtained with Rutin (5) (IC50=1.6 µM). Cytotoxicity was also made. These findings demonstrate that the leaves of A. eliocarpus are a reach source of flavonoids with potential antileismanial and antiplasmodial properties. Investigations of this species are in progress for other medicinal properties.