Chemical composition, antioxidant and antimicrobial activity of guavirova (Campomanesia xanthocarpa Berg) seed extracts obtained by supercritical CO2 and compressed n-butane

Abstract This work reports the chemical characterization, antioxidant and antimicrobial activity of Campomanesia xanthocarpa seed extracts obtained from compressed n -butane and supercritical CO 2 extraction. For supercritical CO 2 , the extraction condition was 40 °C and 250 bar, while for n -butane 35 °C and 10 bar. The total phenolic compound content was 68.58 ± 1.15 mg g −1 for n -butane extract and 17.18 ± 2.44 mg g −1 for supercritical CO 2 . Both extracts were rich in terpenoids, especially β-caryophyllene, while n -butane extract had a larger content of flavonoids than supercritical CO 2 . The antioxidant activity was tested with FRAP, DPPH and deoxyribose assay, with n -butane extracts exhibiting better results. Antimicrobial activity was verified for both extracts against Pseudomonas aeruginosa , however, supercritical CO 2 extract afforded a better performance, reaching 5.0 mg mL −1 for MIC and 10.0 mg mL −1 for MBC, while n -butane extract showed 40.0 mg mL −1 in both antimicrobial tests. Higher extraction yields were obtained using n -butane, which also presented higher antioxidant activity, while supercritical CO 2 extracts afforded the highest antimicrobial activity.