Simultaneous characterization and quantification of flavonoids in Euonymus alatus (Thunb.) Siebold from different origins by HPLC-PAD–MS

Abstract A high-performance liquid chromatography coupled with photodiode array detection and electrospray ionization tandem mass spectrometry (HPLC-PAD–ESI-MS) method has been developed for the simultaneous identification and quantification of active compounds (rutin and quercetin) contained in Traditional Chinese Medicine (TCM) Euonymus alatus ( Thunb .) Siebold (EAS). The herb samples from ten main origins and five medicinal portions (leaf, fruit, stem, pterygium and root) were investigated. The separation was performed on a Shim C 18 column at 30 °C with an isocratic elution. Methanol (A) and water (0.5% methanoic acid, v/v) (B) were used as mobile phases. The recoveries of the two compounds were 100.184% and 100.417%, respectively, and all of them showed good linearity ( r 2  ⩾ 0.9993) in relatively wide concentration ranges. The developed method was applied to identify and quantify the two major active compounds in the collected herb samples, and the results indicated that contents of the two compounds in EAS varied significantly from habitat to habitat. It was demonstrated that the proposed method was helpful for the quality evaluation of EAS.