Preparation method of dihydromyricetin-carried ternary complex liposome

The invention provides a preparation method of dihydromyricetin-carried ternary complex liposome. The preparation method comprises the following steps: (1) preparing an anion liposome ; (2) preparing an anion-cation binary complex liposome suspension; (3) preparing dihydromyricetin ternary complex liposome. According to the dihydromyricetin-carried ternary complex liposome prepared by a layer-by-layer self-assembly technology provided by the invention, an ultrathin membrane is formed by the alternating deposition of substances with opposite charges on a liquid solid interface through an electrostatic interaction, and a brand new multi-layer self-assmebling system: 'anion lipid-cation lipid-carboxymethyl chitosan' is put forward. The ternary complex liposome, of which the particle diameter of is 204.8+/-6.2nm and the potential is -8.1+/-2.5mV after the completion of the assembly, has a significant laminated structure and good stability in a plasma; furthermore, the cytotoxicity is less than cationic liposome; meanwhile, the ternary complex liposome has a pH sensitivity, and is capable of selectively releasing dihydromyricetin in different mediums.