A planarian RPS3 homolog is critical to the modulation of planarian regeneration

Abstract Planarians are an important model for stem cell and regeneration biology, because they can regenerate any missing body structure in a short time. As an important component of ribosomes, ribosomal proteins can synthesize proteins and play a central role in cell cycle checkpoint, cell survival/senescence/apoptosis, and organismal growth and development. In this study, we identified and amplified the homologous gene of RPS3 in Dugesia japonica . Double-stranded RNA mediated RNAi revealed that when the Dj-RPS3 function was lost by planarians; they did not form blastemas and died 100%. Further investigation, confirmed that Dj-RPS3 was involved in regulating the proliferative and early differentiation of neoblasts.