Effect of dental pulp stem cell cultured by three dimensions on reparation of dental injury

Objectine:In the rat dental injury model,3-dimension culture system of dental pulp stem cell(DPSC) affected by BMP-2 was used.The ability of DPSC to the dental injury was observed.Method:The DPSC mass in the 3-dimension culture system was cultured for 7 days after added with BMP-2.The cell mass was marked and identified by Brdu,then was put in the holes of rat dental injury model.Result:Osteoid dentin substance was found in the 3-dimension culture system of DPSC after 4 weeks.No tissue inflammation and necrosis was found..Osteoid dentium cell could be seen in the substance.Dentium cell with high apophysis was developed to the tubiform dentium in the osteoid dentin substance.Conclusion:Injured dental surface implanted by the DPSC mass added with BMP can bring about the reparative dentin.