Dynamics in Kanamycin-Resistant Bacterial Population and Shift of nptII Gene in the Phyllosphere of Insect-Resistant Transgenic Cotton

【Objective】The dynamics in kanamycin-resistant bacterial population were studied and the shift of nptII gene in the phyllosphere of insect-resistant transgenic cotton was monitored. 【Method】Insect-resistant transgenic cotton 33B, SGK321 and GK12, and corresponding receptor cotton 33, Shiyuan 321 and No.3 Simian cotton were sampled at different growing stages. Dynamics and diversity among kanamycin-resistant bacterial populations in the phyllosphere were analyzed with the conventional culture methods. In addition, the shift of nptII gene was monitored by germ PCR, and the unique primer was designed from the sequence of nptⅡ gene, the CK is pBI121 plasmids【.Result】 At different sample times, significant difference was found among the total number and diversity index of phyllospere kanamycin-resistant bacterial population in each cotton variety, but little difference was found between Insect-resistant transgenic cotton and corresponding receptor cotton at the same time. A positive segment was discovered from the kanamycin-resistant bacteria in the phyllosphere of insect-resistant transgenic cotton SKG321, GK12 and receptor cotton No.3 Simian cotton【.Conclusion】It is suggested that little difference was found among the total number and diversity index of phyllospere kanamycin-resistant bacterial population between Insect-resistant transgenic cotton and corresponding receptor cotton,and nptⅡ gene in insect-resistant transgenic cottons could drift into the phyllosphere bacteria.